Caspase Alone Can Induce DNA Fragmentation In Isolated Nuclei
- 주제(KDC) 400
- 개최기관명 이화여자대학교 세포신호전달연구센터
- 발표년도 2000
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000059833
- 본문언어 영어
초록/요약
Apoptosis is a programmed cell death characterized by the cellular and nuclear changes including chromosomal DNA fragmentation(DF). For the nuclear DF the activation of pro-caspase to processed caspase, an apoptosis-specific cysteine protease, is required. The protease alone, however, was known to be unable to induce the DF in nucleus(reviewed in Nature, 401:127) and cellular factor(s) such as DFF40/CAD was reported to be necessay. In this study, however, it is shown that the DF can occur by caspase-3 and 7(but not by 6 and 8) without the aid of any cytoplasmic factors. For the maximal DF 50~75 mM NaCl or KCl is necessary. The DF can be inhibited by ICAD protein, the CAD specific inhibitor. Fractionation of nuclear extract by size-exclusion column and the following Western blotting experiments indicates the involvement of DFF40/CAD-related DNase in the process.
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