Interleukin-1 alpha, promotes extracellular shedding of syndecan-2 via induction of matrix metalloproteinase-7 expression
- 주제(키워드) Interleukin , Syndecan , Matrix metalloproteinase , Extracellular matrix
- 등재 SCIE, SCOPUS
- 발행기관 ACADEMIC PRESS INC ELSEVIER SCIENCE
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000090974
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bbrc.2014.02.142
초록/요약
The cell surface heparan sulfate proteoglycan, syndecan-2, is known to play an important role in the tumorigenic activity of colon cancer cells. In addition, the extracellular domain of syndecan-2 is cleaved by matrix metalloproteinase-7 (MMP-7) in various colon cancer cells, but factors involved in regulating this process remain unknown. Here, we demonstrate a role for interleukin-lot (IL-1 alpha) in syndecan-2 shedding in colon cancer cells. Treatment of low metastatic (HT-29) and highly metastatic (HCT-116) colon cancer cells with various soluble growth factors and cytokines revealed that IL-1 alpha specifically increased extracellular shedding of syndecan-2 in a concentration- and time-dependent manner. IL-1 alpha did not affect the expression of syndecan-2, but did significantly reduce its cell surface levels. Notably, IL-1 alpha increased the mRNA expression and subsequent secreted levels of MMP-7 protein and enhanced the phosphorylation of p38 and ERK mitogen-activated protein kinases. Furthermore, increased syndecan-2 shedding was dependent on the mitogen-activated protein kinase-mediated MMP-7 expression. Taken together, these data suggest that IL-1 alpha regulates extracellular domain shedding of syndecan-2 through regulation of the MAP kinase-mediated MMP-7 expression in colon cancer cells. (C) 2014 Elsevier Inc. All rights reserved.
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