Biosynthesis of glycosylated derivatives of tylosin in Streptomyces venezuelae
- 주제(키워드) Combinatorial biosynthesis , Streptomyces venezuelae , Tylosin
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 Springer Verlag
- 발행년도 2011
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000092402
- 본문언어 영어
- Published As http://dx.doi.org/10.4014/jmb.1103.03032
초록/요약
Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone. © The Korean Society for Microbiology and Biotechnology.
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