Characterization of FK506 biosynthetic intermediates involved in post-PKS elaboration
- 등재 SCIE, SCOPUS
- 발행기관 American Chemical Society
- 발행년도 2013
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000095261
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/np4001224
초록/요약
The post-PKS modification steps of FK506 biosynthesis include C9-oxidation and 31-O-methylation, but the sequence of these reactions and the exact route have remained unclear. This study details the post-PKS modification pathways in FK506 biosynthesis through the identification of all intermediates and in vitro enzymatic reactions of the cytochrome P450 hydroxylase FkbD and the methyltransferase FkbM. These results complete our understanding of post-PKS modification steps to FK506 showing the substrate flexibility of two enzymes involved and the existence of two parallel biosynthetic routes to FK506. © 2013 The American Chemical Society and American Society of Pharmacognosy.
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