Apocynin regulates cytokine production of CD8+ T cells
- 주제(키워드) Apocynin , NOX2 , TNF-α
- 등재 SCIE, SCOPUS
- 발행기관 Springer-Verlag Italia s.r.l.
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000098586
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s10238-013-0241-x
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Apocynin is known to suppress the production of reactive oxygen species (ROS) by inhibiting NADPH oxidases, specifically phagocytic NADPH oxidase (PHOX or NOX2). Given the pro-inflammatory effects of ROS, apocynin has been studied extensively for its use as a therapeutic agent in various disease models. While the effects of apocynin on neutrophils and monocytes have been investigated, it remains to be elucidated whether apocynin modulates the effector function of T cells. In the present study, we examined the effect of apocynin on CD8 + T cells and further investigated its mechanism of action. We found that apocynin directly inhibited the production of pro-inflammatory cytokines such as TNF-α, IFN-γ, and IL-2 in anti-CD3/anti-CD28-stimulated CD8+ T cells. The action of apocynin was upstream of the protein kinase C and calcium signaling in the T cell receptor signaling pathway because apocynin did not inhibit cytokine production in phorbol 12-myristate 13-acetate/ionomycin-stimulated CD8+ T cells. Electrophoretic mobility shift assays revealed that apocynin attenuated anti-CD3/anti-CD28- induced NF-κB activation in CD8+ T cells. In the experiments with NOX2-deficient mice, we demonstrated that apocynin inhibited TNF-α production of CD8+ T cells in a NOX2-independent manner. Taken together, we demonstrated that apocynin, a well-known NOX2 inhibitor, suppressed the cytokine production of CD8+ T cells. We also showed the NOX2-independent action of apocynin in the inhibition of TNF-α production in CD8+ T cells. © 2013 Springer-Verlag.
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