Characterization of fortimicin aminoglycoside profiles produced from Micromonospora olivasterospora DSM 43868 by high-performance liquid chromatography-electrospray ionization-ion trap-mass spectrometry
- 주제(키워드) Chiral column , Fortimicin aminoglycosides , HPLC-ESI–ion trap–MS/MS , Micromonospora olivasterospora , Pentafluoropropionic acid
- 등재 SCIE, SCOPUS
- 발행기관 Springer Verlag
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000123355
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s00216-015-9281-2
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
In this study, an efficient high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)–ion trap-tandem mass spectrometry (MS/MS) was developed for the identification of the biosynthetic congeners involved in the aminocyclitol aminoglycosidic fortimicin pathway from Micromonospora olivasterospora fermentation. The usage of both acid extraction (pH ∼2.5) followed by an cationic-exchanging SPE cleanup and pentafluoropropionic acid mediated ion-pairing chromatography with ESI–ion trap–MS/MS detection was determined to be sufficiently practical to profile the fortimicin (FOR) congeners produced in a culture broth. The limit of the quantification for the fortimicin A (FOR-A) standard spiked in the culture broth was ∼1.6 ng mL−1. The average recovery rate was 93.6 %, and the intra- and inter-day precisions were <5 % with accuracy in the range from 87.1 to 94.2 %. Moreover, the epimeric mixtures including FOR-KH, FOR-KR, and FOR-B were separately resolved through a macrocyclic glycopeptide (teicoplanin)-bonded chiral column. As a result, ten natural FOR pseudodisaccharide analogs were identified and semi-quantified in descending order as follows: FOR-A, FOR-B, DCM, FOR-KH plus FOR-KR, FOR-KK1, FOR-AP, FOR-KL1, FOR-AO, and FOR-FU-10. This is the first report on both the simultaneous characterization of diverse structurally closely related FORs derived from bacterial fermentation using HPLC-ESI–ion trap–MS/MS analysis and the chromatographic separation of the three FOR epimers. [Figure not available: see fulltext.] © 2016 Springer-Verlag Berlin Heidelberg
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