Interspecies complementation of the LuxR family pathway-specific regulator involved in macrolide biosynthesis
- 주제(키워드) Interspecies complementation , LAL transcriptional regulator , Pikromycin , Polyketide , Streptomyces venezuelae
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 Korean Society for Microbiolog and Biotechnology
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000123863
- 본문언어 영어
- Published As http://dx.doi.org/10.4014/jmb.1510.10085
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
PikD is a widely known pathway-specific regulator for controlling pikromycin production in Streptomyces venezuelae ATCC 15439, which is a representative of the large ATP-binding regulator of the LuxR family (LAL) in Streptomyces sp. RapH and FkbN also belong to the LAL family of transcriptional regulators, which show greatest homology with the ATP-binding motif and helix-turn-helix DNA-binding motif of PikD. Overexpression of pikD and heterologous expression of rapH and fkbN led to enhanced production of pikromycin by approximately 1.8-, 1.6-, and 1.6-fold in S. venezuelae, respectively. Cross-complementation of rapH and fkbN in the pikD deletion mutant (ΔpikD) restored pikromycin and derived macrolactone production. Overall, these results show that heterologous expression of rapH and fkbN leads to the overproduction of pikromycin and its congeners from the pikromycin biosynthetic pathway in S. venezuelae, and they have the same functionality as the pathwayspecific transcriptional activator for the pikromycin biosynthetic pathway in the ΔpikD strain. These results also show extensive “cross-communication” between pathway-specific regulators of streptomycetes and suggest revision of the current paradigm for pathwayspecific versus global regulation of secondary metabolism in Streptomyces species. © 2016 by The Korean Society for Microbiology and Biotechnology.
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