Galangin Suppresses Pro-Inflammatory Gene Expression in Polyinosinic-Polycytidylic Acid-Stimulated Microglial Cells
- 주제(키워드) Galangin , Poly(I:C) , Microglia , PI3K/Akt , NF-kappa B , PPAR-gamma signaling
- 주제(기타) Biochemistry & Molecular Biology; Pharmacology & Pharmacy
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC APPLIED PHARMACOLOGY
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000149647
- 본문언어 영어
- Published As http://dx.doi.org/10.4062/biomolther.2017.173
초록/요약
Galangin (3,5,7-trihydroxyflavone) is a polyphenolic compound abundant in honey and medicinal herbs, such as Alpinia officinarum. In this study, we investigated the anti-inflammatory effects of galangin under in vitro and in vivo neuroinflammatory conditions caused by polyinosinic-polycytidylic acid (poly(I:C)), a viral mimic dsRNA analog. Galangin suppressed the production of nitric oxide, reactive oxygen species, and pro-inflammatory cytokines in poly(I:C)-stimulated BV2 microglia. On the other hand, galangin enhanced anti-inflammatory interleukin (IL)-10 production. Galangin also suppressed the expression of pro-inflammatory markers in poly(I:C)-injected mouse brains. Further mechanistic studies showed that galangin inhibited poly(I:C)-induced nuclear factor (NF)-kappa B activity and phosphorylation of Akt without affecting MAP kinases. Interestingly, galangin increased the expression and transcriptional activity of peroxisome proliferator-activated receptor (PPAR)-gamma, known to play an anti-inflammatory role. To investigate whether PPAR-gamma is involved in the anti-inflammatory function of galangin, BV2 cells were pre-treated with PPAR-gamma antagonist before treatment of galangin. We found that PPAR-gamma antagonist significantly blocked galangin-mediated upregulation of IL-10 and attenuated the inhibition of tumor necrosis factor (TNF)-alpha and IL-6 in poly(I:C)-stimulated microglia. In conclusion, our data suggest that PI3K/Akt, NF-kappa B, and PPAR-gamma play a pivotal role in mediating the anti-inflammatory effects of galangin in poly(I:C)-stimulated microglia.
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