Preventive effects of tonsil-derived mesenchymal stem cells on osteoradionecrosis in a rat model
- 주제(키워드) animal model , cell therapy , mesenchymal stem cell , osteoradionecrosis , tonsils
- 등재 SCIE, SCOPUS
- 발행기관 John Wiley and Sons Inc.
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000149888
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/hed.25004
초록/요약
Background: The purpose of this study was to investigate the effects of tonsil-derived mesenchymal stem cells (MSCs) on osteoradionecrosis (ORN). Method: We generated a mandibular ORN rat model using a combination of 20-Gy single-dose irradiation and tooth extraction. Study groups were negative control (tooth extraction only), ORN group (irradiation, tooth extraction), Matrigel-1 group (Matrigel; BD Biosciences, San Jose, CA; irradiation, Matrigel application immediately after tooth extraction), tonsil-derived MSC-1 group (irradiation, tonsil-derived MSC application immediately after tooth extraction), Matrigel-4 group (irradiation, Matrigel application 4 weeks after tooth extraction), and tonsil-derived MSC-4 group (irradiation, tonsil-derived MSC application 4 weeks after tooth extraction). Result: Bone mineral density was significantly lower in the ORN group than in the negative control group. The tonsil-derived MSC-1 group showed significantly higher bone mineral density than did the ORN and tonsil-derived MSC-4 groups. Conclusion: A single 20-Gy dose of irradiation combined with tooth extraction successfully generated ORN in the rat model. The tonsil-derived MSCs can be effective for bone regeneration in ORN, particularly when applied immediately after dentoalveolar trauma or surgery. © 2017 Wiley Periodicals, Inc.
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