Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities
- 주제(키워드) Polypyridyl ruthenium complex , Cytotoxic activity , Cellular uptake , Anticancer activity , Cell migration
- 주제(기타) Biochemistry & Molecular Biology; Chemistry, Inorganic & Nuclear
- 설명문(일반) [Tikum, Anjong Florence; Jeon, Yu Jeong; Lee, Ju Hyun; Baea, In Yeong; Kim, Jinheung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 03760, South Korea; [Park, Min Hee; Baea, In Yeong; Kim, Sang Heon] Korea Inst Sci & Technol, Ctr Biomat, Seoul, South Korea; [Kim, Sang Heon] Univ Sci & Technol, Dept Biomed Engn, Daejeon, South Korea; [Lee, Hye Jin] Kyungpook Natl Univ, Dept Chem, 80 Daehakro, Daegu 41566, South Korea; [Lee, Hye Jin] Kyungpook Natl Univ, Green Nano Mat Res Ctr, 80 Daehakro, Daegu 41566, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE INC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151581
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jinorgbio.2018.01.003
초록/요약
Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)(2)](2+) (1), [(piq)Ru(phen)(2)](2+) (2), and [(piq)Ru(DIP)(2)](2+). (3) (piq = phenylisoquinolinate, bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-dipheny1-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1-3 to double-stranded DNA were studied. The binding of 1-3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1-3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1-3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1-3 on the survival of MDA-MB-231 cells were examined and compared with that of cisplatin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1-3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1-3 was also evaluated by the wound healing assay.
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