Synergistic effects of sanghuang–danshen bioactives on arterial stiffness in a randomized clinical trial of healthy smokers: An integrative approach to in silico network analysis
- 주제(키워드) Arterial stiffness , Danshen , Endothelium-dependent vasodilation , In silico network analysis , Integrative approach , Randomized clinical trial , Sanghuang mushroom
- 등재 SCIE, SCOPUS
- OA유형 Green Published, Green Submitted, gold
- 발행기관 MDPI AG
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000156329
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/nu11010108
- PubMed https://pubmed.ncbi.nlm.nih.gov/30621047
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The vascular endothelium is a favorite early target of cardiovascular risk factors, including cigarette smoking. Here, we investigated the synergistic effects of Sanghuang–Danshen (SD) bioactives on vascular stiffness in a controlled clinical trial of healthy chronic smokers (n = 72). Relative to placebo, 4-week SD consumption at 900 mg/day improves pulse wave velocity (p = 0.0497), reduces systolic blood pressure (peripheral, p = 0.0008; brachial, p = 0.0046; and ankle, p = 0.0066), and increases endothelial nitric oxide synthase activation (p < 0.0001). We then mapped all differential markers obtained from the clinical data, Affymetrix microarray, and1 H NMR metabolomics, together with 12 SD bioactives, onto the network platform termed the context-oriented directed associations. The resulting vascular subnetwork demonstrates that ellagic acid, caffeic acid, protocatechuic acid, cryptotanshinone, tanshinone I, and tanshinone IIA are linked to NOS3, ARG2, and EDN1 for vascular dilation, implicated with arginine/proline metabolism. They are also linked to SUCLG1, CYP1A1, and succinate related to the mitochondrial metabolism and detoxification, implicated with various metabolic pathways. These results could explain the synergistic action mechanisms of SD bioactives in the regulation of vascular endothelial dilation and metabolism, confirming the potential of SD in improving vascular stiffness and blood pressure in healthy smokers. © 2019, MDPI AG. All rights reserved.
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