Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
- 주제(키워드) Codon optimization , Corynebacterium glutamicum , davTDBA , Fed-batch fermentation , Glutaric acid , His6-tag , L‐Lysine
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 Academic Press Inc.
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000156905
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.ymben.2018.08.007
- PubMed https://pubmed.ncbi.nlm.nih.gov/30144560
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. It was found that use of N-terminal His6-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation. © 2018 International Metabolic Engineering Society
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