Extracellular Vesicles Derived from Lactobacillus plantarum Increase BDNF Expression in Cultured Hippocampal Neurons and Produce Antidepressant-like Effects in Mice
- 주제(키워드) Extracellular vesicles , Lactobacillus plantarum , BDNF , Antidepressant-like effects
- 주제(기타) Medicine, Research & Experimental; Neurosciences
- 설명문(일반) [Choi, Juli; Han, Pyung-Lim] Ewha Womans Univ, Dept Brain & Cognit Sci, Seoul 03760, South Korea; [Kim, Yoon-Keun] MD Healthcare Inc, Seoul 03923, South Korea; [Han, Pyung-Lim] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Submitted, gold, Green Published
- 발행기관 KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159865
- 본문언어 영어
- Published As http://dx.doi.org/10.5607/en.2019.28.2.158
- PubMed https://pubmed.ncbi.nlm.nih.gov/31138987
초록/요약
Gut microbiota play a role in regulating mental disorders, but the mechanism by which gut microbiota regulate brain function remains unclear. Gram negative and positive gut bacteria release membrane-derived extracellular vesicles (EVs), which function in microbiota-host intercellular communication. In the present study, we investigated whether Lactobacillus plantarum derived EVs (L-EVs) could have a role in regulating neuronal function and stress-induced depressive-like behaviors. HT22 cells treated with the stress hormone glucocorticoid (GC; corticosterone) had reduced expression of Bdnf and Sirt1, whereas L -EV treatment reversed GC-induced decreased expression of Bdnf and Sirt1. The siRNA-mediated knockdown of Sirt1 in HT22 cells decreased Bdnf4, a splicing variant of Bdnf, and Creb expression, suggesting that Sirt1 plays a role in L -EV-induced increase of BDNF and CREB expression. Mice exposed to restraint for 2-h daily for 14 days (CRST) exhibited depressive-like behaviors, and these CRST-treated mice had reduced expression of Bdnf and Nt4/5 in the hippocampus. In contrast, L-EV injection prior to each restraint treatment blocked the reduced expression of Bdnf and Nt4/5, and stress-induced depressive-like behaviors. Furthermore, L-EV treatment in CRST-treated mice also rescued the reduced expression of Bdnf, and blocked stress-induced depressive-like behaviors. These results suggest that Lactobacillus derived EVs can change the expression of neurotropic factors in the hippocampus and afford antidepressant-like effects in mice with stress-induced depression.
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