Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
- 주제(키워드) urinary cell DNA , liquid biopsy , commercial kit
- 주제(기타) Medicine, General & Internal
- 설명문(일반) [Lee, Eun Young; Lee, Eun-Ju; Yoon, Hana; Lee, Dong Hyeon; Kim, Kwang Hyun] Ewha Womans Univ, Dept Urol, Coll Med, Seoul 07804, South Korea; [Lee, Eun Young; Lee, Eun-Ju] Ewha Womans Univ, Ewha Med Res Inst, Coll Med, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS
- OA유형 Green Published
- 발행기관 MDPI
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000169228
- 본문언어 영어
- Published As https://dx.doi.org/10.3390/diagnostics10040234
- PubMed https://pubmed.ncbi.nlm.nih.gov/32325682
초록/요약
Urinary cell-free DNA (cfDNA) is an attractive body fluid for liquid biopsy. In this study, we compared the efficiencies of four commercial kits for urinary cell-free DNA (cfDNA) isolation and of various sample storage conditions. Urinary cfDNA was isolated from 10 healthy individuals using four commercial kits: QIAamp Circulating Nucleic Acid Kit (QC; Qiagen), MagMAX (TM) Cell-Free DNA Isolation Kit (MM; Applied Biosystems), Urine Cell-Free Circulating DNA Purification Midi Kit (NU; Norgen Biotek), and Quick-DNA (TM) Urine Kit (ZQ; Zymo Research). To assess the isolation efficiency, an Agilent 2100 Bioanalyzer with High Sensitivity DNA chips was used, and cfDNA yield was defined as the amount of cfDNA obtained from 1 mL of urine. MM and QC provided the highest cfDNA yield in the 50-300 bp range, and MM and NU gave the highest cfDNA yield in the 50-100 bp range. In particular, the NU kit was efficient for isolation of more fragmented cfDNA in the range of 50-100 bp with the lowest cellular genomic DNA contamination. ZQ had the best cost-efficiency for isolating the same amount of urinary cfDNA. Samples stored at -70 degrees C with the addition of 10 mM EDTA resulted in the highest cfDNA yield 3 months after sample collection.
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