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Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model

  • 주제(키워드) Stem cells , Tonsil , Oral mucositis , Wound healing , Mechanical trauma
  • 주제(기타) Cell & Tissue Engineering
  • 주제(기타) Engineering, Biomedical
  • 설명문(일반) [Jung, Harry; Park, Hae Sang] Hallym Univ, Hallym Clin & Translat Sci Inst, Inst New Frontier Res Team, 1 Hallymdaehak Gil, Chunchon 24252, Gangwon Do, South Korea; [Kim, Han Su] Ewha Womans Univ, Sch Med, Dept Otorhinolaryngol Head & Neck Surg, 1071 Anyangcheon Ro, Seoul 07985, South Korea; [Lee, Jun Ho; Park, Hae Sang] Hallym Univ, Chuncheon Sacred Heart Hosp, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, 77 Sakju Ro, Chunchon 24253, Gangwon Do, South Korea; [Lee, Jae Jun] Hallym Univ, Coll Med, Dept Anesthesiol & Pain Med, 77 Sakju Ro, Chunchon 24253, Gangwon Do, South Korea
  • 등재 SCIE, SCOPUS, KCI등재
  • OA유형 Green Published
  • 발행기관 KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
  • 발행년도 2020
  • 총서유형 Journal
  • URI http://www.dcollection.net/handler/ewha/000000169259
  • 본문언어 영어
  • Published As https://dx.doi.org/10.1007/s13770-019-00226-7
  • PubMed https://pubmed.ncbi.nlm.nih.gov/32002842

초록/요약

Background: We first determined the efficacy of lesional injection of tonsil-derived MSCs (mesenchymal stem cells) for the treatment of 5-fluorouracil induced oral mucositis. Methods: Oral mucositis was induced in hamsters by administration of 5-fluorouracil (day 0, 2, 4) followed by mechanical trauma (day 1, 2, 4). The experimental groups included MT (mechanical trauma only), 5-FU + MT (mechanical trauma with 5-fluorouracil administration), TMSC (mechanical trauma with 5-fluorouracil administration, tonsil-derived mesenchymal stem cells injection), DEXA (mechanical trauma with 5-fluorouracil administration, dexamethasone injection), and saline (mechanical trauma with 5-fluorouracil administration, saline injection). Results: On day 10, gross and histologic analyses showed that nearly complete healing and epithelialization of the cheek mucosa of the TMSC group, whereas the other groups showed definite ulcerative lesions. Compared with the MT and DEXA groups, CD31 expression was greater in the TMSC group on days 10 and 14. Tendency towards a decrease in MMP2 expression with the time in the TMSC group was observed. In addition, the TMSC group showed higher expression of TGF-beta, and NOX4 on day 10 compared with the other groups. Scratch assay demonstrated that the conditioned media harvested from tonsil-derived MSCs significantly increased migratory efficacy of NIH3T3 cells. Transwell assay showed that the preferential migration of tonsil-derived MSCs to the wound area. Conclusion: Intralesional administration of tonsil-derived MSCs may accelerate wound healing of 5-fluorouracil induced oral mucositis by upregulating neovascularization and effective wound contraction. In addition, tonsil-derived MSCs might contribute to oral ulcer regeneration via the stimulation of fibroblast proliferation and migration.

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