Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation
- 주제(키워드) Angioimmunoblastic T-cell , lymphoma , RHOA , follicular helper T-cell , PD-1
- 주제(기타) Oncology
- 주제(기타) Immunology
- 설명문(일반) [Lee, Gyu Jin; Jun, Yukyung; Lee, Daekee; Lee, Sanghyuk; Kim, Jaesang] Ewha Womans Univ, Dept Life Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Lee, Gyu Jin; Jun, Yukyung; Lee, Daekee; Lee, Sanghyuk; Kim, Jaesang] Ewha Res Ctr Syst Biol, Seoul, South Korea; [Yoo, Hae Yong] Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol, Dept Hlth Sci & Technol, Seoul, South Korea; [Jeon, Yoon Kyung] Seoul Natl Univ, Dept Pathol, Coll Med, Seoul, South Korea; [Jeon, Yoon Kyung] Seoul Natl Univ, Canc Res Inst, Seoul, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 TAYLOR & FRANCIS INC
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000174604
- 본문언어 영어
- Published As http://dx.doi.org/10.1080/2162402X.2020.1746553
- PubMed https://pubmed.ncbi.nlm.nih.gov/32923110
초록/요약
A missense mutation in RHOA encoding p.Gly17 Val has been reported to occur frequently in angioimmunoblastic T-cell lymphoma (AITL). Here, we describe a murine model which expresses the human RHOA mutant gene product in a T-cell specific manner and develops AITL-like symptoms. Most transgenic mice feature with latency one or two enlarged lymph nodes characterized by aberrant lymph node architecture, extensive lymphocytic infiltration, extrafollicular meshwork of follicular dendritic cells (FDC) and arborized endothelial venules. Importantly, we provide evidence for expansion of PD-1 + follicular helper T (Tfh) cells which are the neoplastic cells of AITL. In addition, we saw proliferation of B-cells leading to hypergammaglobulinemia and the presence of dominant T cell clonal populations. Transplantation of lymph node cells to immunocompromised mice partly recreated lymphadenopathy after a long latency and with low penetrance suggesting that cells have undergone partial transformation to a premalignant state. Transcriptomic profiling revealed that the gene expression pattern within affected lymph nodes of the mice closely resembles that of AITL patients with the identical RHOA p.Gly17 Val mutation. The murine model should, therefore, be useful in dissecting pathogenesis of AITL at the molecular level particularly for the cases with the RHOA p.Gly17Val mutation.
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