Co-transplantation of tonsil-derived mesenchymal stromal cells in bone marrow transplantation promotes thymus regeneration and T cell diversity following cytotoxic conditioning
- 주제(키워드) tonsil-derived mesenchymal stromal cells , bone marrow transplantation , thymus , T cell diversity
- 주제(기타) Medicine, Research & Experimental
- 설명문(일반) [Choi, Da-Won; Cho, Kyung-Ah; Lee, Hyun-Ji; Kim, Yu-Hee; Woo, So-Youn] Ewha Womans Univ, Coll Med, Dept Microbiol, 25 Magokdong Ro 2 Gil, Seoul 07804, South Korea; [Woo, Kyong-Je] Ewha Womans Univ, Coll Med, Dept Plast & Reconstruct Surg, Seoul 07804, South Korea; [Park, Joo-Won] Ewha Womans Univ, Coll Med, Dept Biochem, Seoul 07804, South Korea; [Ryu, Kyung-Ha] Ewha Womans Univ, Coll Med, Dept Pediat, Seoul 07804, South Korea
- 등재 SCIE, SCOPUS
- OA유형 hybrid, Green Published
- 발행기관 SPANDIDOS PUBL LTD
- 발행년도 2020
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000175745
- 본문언어 영어
- Published As http://dx.doi.org/10.3892/ijmm.2020.4657
- PubMed https://pubmed.ncbi.nlm.nih.gov/32582998
초록/요약
Bone marrow (BM) transplantation (BMT) represents a curative treatment for various hematological disorders. Prior to BMT, a large amount of the relevant anticancer drug needed to be administered to eliminate cancer cells. However, during this pre-BMT cytotoxic conditioning regimen, hematopoietic stem cells in the BM and thymic epithelial cells were also destroyed. The T cell receptor (TCR) recognizes diverse pathogen, tumor and environmental antigens, and confers immunological memory and self-tolerance. Delayed thymus reconstitution following pre-BMT cytotoxic conditioning impedesde novothymopoiesis and limits T cell-mediated immunity. Several cytokines, such as RANK ligand, interleukin (IL)-7, IL-22 and stem cell factor, were recently reported to improve thymopoiesis and immune function following BMT. In the present study, it was found that the co-transplantation of tonsil-derived mesenchymal stromal cells (T-MSCs) with BM-derived cells (BMCs) accelerated the recovery of involuted thymuses in mice following partial pre-BMT conditioning with busulfan-cyclophosphamide treatment, possibly by inducing FMS-like tyrosine kinase 3 ligand (FLT3L) and fibroblast growth factor 7 (FGF7) production in T-MSCs. The co-transplantation of T-MSCs with BMCs also replenished the CD3(+)cell population by inhibiting thymocyte apoptosis following pre-BMT cytotoxic conditioning. Furthermore, T-MSC co-transplantation improved the recovery of the TCR repertoire and led to increased thymus-generated T cell diversity.
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