Production of human spinal-cord organoids recapitulating neural-tube morphogenesis
- 등재 SCIE, SCOPUS
- OA유형 Green Submitted
- 발행기관 Nature Research
- 발행년도 2022
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000190331
- 본문언어 영어
- Published As https://doi.org/10.1038/s41551-022-00868-4
- PubMed https://pubmed.ncbi.nlm.nih.gov/35347276
초록/요약
Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects. © 2022, The Author(s), under exclusive licence to Springer Nature Limited.
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