Snapin이 신경전달에 주는 분자생물학적 영향 연구
The study of molecular mechanism of Snapin in neurotransmission
- 주제(키워드) Snapin , Dysbindin , DTNBP1 , neurotransmission , Schizophrenia , SNARE
- 발행기관 포항공과대학교 일반대학원
- 지도교수 김기현
- 발행년도 2012
- 학위수여년월 2012. 2
- 학위명 석사
- 학과 및 전공 일반대학원 융합생명공학부
- 세부전공 생물리학
- 원문페이지 38
- 파일정보 pdf
- 실제URI http://www.dcollection.net/handler/postech/000001216441
- 본문언어 영어
- 저작권 포항공과대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Snapin was identified by Zu-Hang Sheng group in 1999 as not only SNAP25 binding protein, but also modulation factor between SNARE(Soluble N-ethyl maleimide sensitive factor Attachment protein REceptor) and Synaptotagmin. But, the definite function of Snapin in neuron still has been debated by many groups of scientists. So, we indirectly verified whether that Snapin can interact with SNARE by means of in vitro vesicle reconstitution methods. Interestingly, The effect of Snapin, which has many positive amino acid residues in their protein sequence, in vesicle Docking/fusion was significantly reduced by negative charged lipid like PS(Phosphatidyl Serine). S50D mutant, which shows mimic effect of S50 phosphorylation by PKA(cAMP-dependent Protein kinase A) showed relived inhibition of PS. On the basis of our results, we insist that Snapin closely interact with SNARE and S50 phosphorylation of Snapin by cAMP depedent PKA could act as docking signal, which may be link between secondary molecule like cAMP and SNARE-mediated membrane fusion.
more목차
Abstract ---------------------------------------------------------------- I
Contents ---------------------------------------------------------------- III
Abbreviations ---------------------------------------------------------- IV
List of figures and tables ---------------------------------------------- VI
Introduction ------------------------------------------------------------ 1
Materials and Methods ----------------------------------------------- 4
Results ------------------------------------------------------------------ 9
Discussion -------------------------------------------------------------- 14
Figures ----------------------------------------------------------------- 17
국문요약 -------------------------------------------------------------- 31
References ------------------------------------------------------------- 32
Acknowledgements -------------------------------------------------- 36
Curriculum vitae ------------------------------------------------------ 37