대장암에서 노화 종양세포 특성에 관한 연구
Study on the Characteristics of Senescent Tumor Cells in the Colorectal Cancers
- 주제(키워드) Cellular senescence , Senescent tumor cell , Colorectal cancer
- 발행기관 아주대학교
- 지도교수 김수동
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 글로벌제약임상대학원 글로벌제약임상약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000027675
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cellular senescence is a condition in which cell proliferation is inhibited irreversibly. Cellular senescence can be induced by several factors such as telomere shortening, oxygen stress, UV, or oncogenic stimulation. Among them, oncogene-induced senescence is induced by oncogene overexpression. Oncogene-induced senescence was initially considered as a barrier of cancer progression. Recently, however, oncogene-induced senescence could be found in advanced cancer and may have a positive effect on cancer progression. In present study, we investigated characteristics of senescence cells in colorectal cancers. We examined colorectal cancer tissues and clinicopathological data from 51 patients who underwent colorectal cancer resection at Ajou University Hospital between 2016 and 2017. We performed SA-β-Gal staining and graded according to the degree of staining. The correlation between grade of SA-β-Gal staining and clinicopathological data was analyzed. We also conducted primary culture to investigate the role of tumor senescent cells. We found that three different staining pattern of senescent cells in colorectal cancer tissues. We obtained no statistical results in the correlation between senescent cells and patient’s data. Although there were no statistical significance between senescent tumor cells and parameters of colorectal cancers, we found senescent tumor cells were more frequently present in early stage of colorectal cancers and in small sized colorectal cancers (p=0.054, p=0.070). Our data suggested that the possibility of correlation between senescent tumor cells and pathogenesis of the colorectal cancers is present.
more목차
I.INTRODUCTION 1
II.MATERIAL AND METHOD 3
A.Cases and samples 3
B.Preparation of SA- β -Gal staining reagent 3
C.SA- β -Gal staining and counter staining 4
D.Grading of SA- β -Gal staining 4
E.Double Immunohistochemistry 5
F.Immunohistochemical grading of senescent tumor cells 5
G.KRAS NRAS mutation Testing 6
H.Primary cell culture 6
I.Statistics 7
III.Results 8
A.Characteristics of SA-β-Gal staining in the colorectal cancers 8
B.Grading of SA-β-Gal staining in the colorectal cancers 11
C.Correlation between SA-β-Gal staining and immunohistochemistry 15
D.Correlation between grades of SA-β-Gal staining and the sex and the age of patients 17
E.Grades of SA-β-Gal staining and extents of primary tumor(pT) 19
F.Comparison between grades of SA-β-Gal staining and the degree of spread to regional lymph nodes(pN) 22
G.Correlation between grades of SA-β-Gal staining and the tumor size of patients 24
H.Correlation between grades of SA-β-Gal staining and the oncogene (KRAS, NRAS) of cancer 26
I.Primary cell culture 29
IV.DISCUSSION 34
V.CONCLUSION 38
REFERENCES 39
국문요약 45
