Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide
- 주제(키워드) Docosahexaenoic acid (DHA) , Ethanol Lipozyme RM IM (Rhizomucor miehei) , Near-supercritical carbon dioxide , Tuna oil fatty acids
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE BV
- 발행년도 2014
- URI http://www.dcollection.net/handler/ewha/000000090732
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.supflu.2013.12.024
초록/요약
This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.
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