Increased gamma-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines
- 주제(키워드) Extremely low frequency , combined treatment , gamma-H2AX , ionizing radiation , H2O2
- 등재 SCIE, SCOPUS
- 발행기관 INFORMA HEALTHCARE
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000090903
- 본문언어 영어
- Published As http://dx.doi.org/10.3109/09553002.2014.887866
초록/요약
Purpose : Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be subtle. Therefore, a more sensitive method and systematic research strategy are warranted to evaluate genotoxicity. Materials and methods: We investigated the effect of ELF-MF in combination with ionizing radiation (IR) or H2O2 on the DNA damage response of expression of phosphorylated H2AX (gamma-H2AX) and production of gamma-H2AX foci in non-tumorigenic human cell systems consisting of human lung fibroblast WI-38 cells and human lung epithelial L132 cells. Results: Exposure to a 60-Hz, 2 mT ELF-MF for 6 h produced increased gamma-H2AX expression, as well as gamma-H2AX foci production, a common DNA double-strand break (DSB) marker. However, exposure to a 1 mT ELF-MF did not have the same effect. Moreover, 2 mT ELF-MF exposure potentiated the expression of gamma-H2AX and gamma-H2AX foci production when combined with IR, but not when combined with H2O2. Conclusions: ELF-MF could affect the DNA damage response and, in combination with different stimuli, provide different effects on gamma-H2AX.
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