Decreased Cellular Levels of Palmitic Amide are Linked to 5-fluorouracil Resistance in Human Colon Cancer Cells
- 주제(키워드) Palmitic amide , 5-fluorouracil , Colon cancer , Proteomics
- 설명문(URI) http://www.ncbi.nlm.nih.gov/pubmed/24901137
- 등재 SCIE, SCOPUS
- 발행기관 H G E UPDATE MEDICAL PUBLISHING S A
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000090995
- 본문언어 영어
초록/요약
Background/Aims: The aim of this study was to investigate whether profiling metabolic compounds in human colon cancer cells with induced 5-florouracil resistance enables identification of predictive bio-markers for 5-florouracil resistance. Methodology: 5-florouracil resistant and parental cells were extracted using methanol/chloroform solution, and analyzed by MALDI-TOF. Principal components analysis and discriminant analysis was performed to select low-mass ions with strong discriminating power between 5-florouracil resistant and parental cells. The correlation between the intensities of low-mass ions and intrinsic 5-florouracil resistance in 11 colon cancer cells was analyzed using the Spearman rank coefficient. Results: Eleven low-mass ions had strong discriminating power between 5-florouracil-resistant and parental cells. Of these, the intensity of a low-mass ion with 256.29m/z was negatively correlated with intrinsic 5-florouracil resistance in 11 colon cancer cells (r = -0.6545, P = 0.0338). By searching the H+ adduct with 0.05 m/z tolerance in the Human Metabolome Database, a low-mass ion of 256.29 m/z was identified as palmitic amide. Interestingly, extracellular treatment with palmitic amide reduced 5-florouracil resistance and invasiveness in 5-florouracil-resistant cells. Conclusions: Palmitic amide showed potential not only as a predictor of 5-florouracil resistance, but also for reduction of 5-florouracil resistance in colon cancer cells.
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