Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells
- 주제(키워드) Echinacea purpurea , 3T3-L1 cells , Adipocyte differentiation , Insulin , Dodeca-2(E) , 4(E)-dienoic acid isobutylamide
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 PHARMACEUTICAL SOC KOREA
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000091611
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s12272-013-0251-y
초록/요약
Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor gamma (PPAR gamma) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1-methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPAR gamma and C/EBP alpha in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPAR gamma and C/EBP alpha in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.
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