Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11
- 주제(키워드) Flo8 , LisH motif , Transcriptional activation , Mss11 , FLO1 , FLO11
- 등재 SCIE, SCOPUS
- 발행기관 ACADEMIC PRESS INC ELSEVIER SCIENCE
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000091699
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bbrc.2014.04.161
초록/요약
Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11 and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets. (C) 2014 Elsevier Inc. All rights reserved.
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