Glycosyltransferase and its application to glycodiversification of natural products
- 주제(키워드) Glycosylation , Glycosyltransferase , Glycosyltransferase-A , Glycosyltransferase-B , Secondary metabolites
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 Korean Society of Industrial Engineering Chemistry
- 발행년도 2012
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000093867
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jiec.2012.01.048
초록/요약
Glycosyltransferase (GT) catalyzes the transfer of a sugar moiety to acceptor substrates such as secondary metabolites. The majority of GTs has two structural folds, GT-A and GT-B based on 3-D structural analysis. The limited structural fold diversity is compensated by a highly divergent acceptor binding domain for conferring sufficient substrate promiscuity. Various GTs have been engineered to further enhance the glucose transfer activity and expand substrate promiscuity by error-prone PCR and site-directed mutagenesis. Engineered GT-catalyzed glycosylation will certainly play a key role in the generation of scaffold for new drug discovery and control of drug pharmacokinetics. © 2012 The Korean Society of Industrial and Engineering Chemistry.
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