β-Carotene inhibits neuroblastoma tumorigenesis by regulating cell differentiation and cancer cell stemness
- 주제(키워드) Cancer stem cells , Differentiation , Neuroblastoma , Tumorigenesis , β-Carotene
- 등재 SCIE, SCOPUS
- 발행기관 Academic Press Inc.
- 발행년도 2014
- URI http://www.dcollection.net/handler/ewha/000000098612
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bbrc.2014.07.021
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Neuroblastoma (NB) is the most common extracranial solid cancer in young children and malignant NB cells have been shown to possess cancer stem cell (CSC) characteristics. Thus, the successful elimination of CSCs represents a strategy for developing an effective preventive and chemotherapeutic agent. CSCs are characterized by differentiation and tumorigenicity. β-Carotene (BC) has been associated with many anticancer mechanisms, although the efficacy of BC on CSCs remains unclear. In the present study, the effects of BC on tumor cell differentiation and tumorigenicity was investigated using a xenograft model. Mice were pretreated with BC for 21 days, then received a subcutaneous injection of SK-N-BE(2)C cells. Both tumor incidence and tumor growth were significantly inhibited for mice that received BC supplementation compared to the control group. Treatment with BC has also been shown to induce tumor cell differentiation by up-regulating differentiation markers, such as vimentin, peripherin, and neurofilament. Conversely, BC treatment has been shown to significantly suppress tumor stemness by down-regulating CSC markers such as Oct 3/4 and DLK1. BC treatment also significantly down-regulated HIF1-α expression and its downstream target, vascular endothelial growth factor (VEGF). Taken together, these results suggest that BC is a potential chemotherapeutic reagent for the treatment of NB, and mediates this effect by regulating the differentiation and stemness of CSCs, respectively. © 2014 Elsevier Inc. All rights reserved.
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