Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation
- 주제(키워드) Candida rugosa lipase , Lipase-catalysed esterification , Pine nut oil , Pinolenic acid , Urea complexation
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2015
- URI http://www.dcollection.net/handler/ewha/000000113032
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.foodchem.2014.08.074
초록/요약
Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 degrees C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield. (C) 2014 Elsevier Ltd. All rights reserved.
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