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Effects of granular activated carbon on methane removal performance and methanotrophic community of a lab-scale bioreactor

초록/요약

Two identical lab-scale bioreactor systems were operated to examine the effects of granular activated carbon (GAC) on methane removal performance and methanotrophic community. Both bioreactor systems removed methane completely at a CH4 loading rate of 71.2g-CH4 center dot d(-1) for 17days. However, the methane removal efficiency declined to 88% in the bioreactor without GAC, while the bioreactor amended with GAC showed greater methane removal efficiency of 97% at a CH4 loading rate of 107.5g-CH4 center dot d(-1). Although quantitative real-time PCR showed that methanotrophic populations were similar levels of 5-10 x 10(8) pmoA gene copy number center dot VSS-1 in both systems, GAC addition changed the methanotrophic community composition of the bioreactor systems. Microarray assay revealed that GAC enhanced the type I methanotrophic genera including Methylobacter, Methylomicrobium, and Methylomonas of the system, which suggests that GAC probably provided a favorable environment for type I methanotrophs. These results indicated that GAC is a promising support material in bioreactor systems for CH4 mitigation.

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