Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst
- 주제(키워드) Biocatalysts , Biotransformation , Corynebacterium glutamicum , 10-Ketostearic acid , Oleate hydratase , Oleic acid , Secondary alcohol dehydrogenase
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000114799
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s10529-015-1775-7
초록/요약
To produce 10-ketostearic acid from oleic acid. Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 A degrees C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1). This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
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