A Comparative Study on Hulled Adlay and Unhulled Adlay through Evaluation of Their LPS-Induced Anti-Inflammatory Effects, and Isolation of Pure Compounds
- 주제(키워드) Coix lacryma-jobi , Phenolic compounds , Inducible nitric oxide synthase (iNOS) , Cyclooxygenase-2 (COX-2)
- 등재 SCIE, SCOPUS
- 발행기관 WILEY-V C H VERLAG GMBH
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000115002
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/cbdv.201400242
초록/요약
Coicis semen (=the hulled seed of Coix lacryma-jobi L. var. ma-yuen (Rom.Caill.) Stapf; Gramineae), commonly known as adlay and Job's tears, is widely used in traditional medicine and as a nutritious food. Bioassay-guided fractionation of the AcOEt fraction of unhulled adlays, using measurement of nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells, led to the isolation and identification of two new stereoisomers, (+)-(7S,8R,7S,8R)-guaiacylglycerol -O-4-dihydrodisinapyl ether (1) and (+)-(7S,8R,7R,8R)-guaiacylglycerol -O-4-dihydrodisinapyl ether (2), together with six known compounds, 3-8. Compounds 3 and 4 exhibited inhibitory activities on LPS-induced NO production with IC50 values of 1.4 and 3.7M, respectively, and suppressed inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions in RAW 264.7 macrophage cells. Simple high-performance liquid chromatography with ultraviolet detection (HPLC/UV) was used to compare the AcOEt fraction of unhulled adlays responsible for the anti-inflammatory activity in RAW 264.7 cells and the inactive AcOEt fraction of hulled adlays.
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