One-Photon and Two-Photon Sensing of Biothiols Using a Bis-Pyrene-Cu(II) Ensemble and Its Application To Image GSH in the Cells and Tissues
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000115006
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/ac504372w
초록/요약
Glutathione (GSH), cysteine (Cys), and homodysteine (Hcy) are three major biothiols, which play key roles in various biological systems. Accordnigly, the development of imaging probes has been actively studied. We report a new pyrene derivative 1, which showed large fluorescence quenching with Cu2+ at pH 7.4. The ensemble 1-Cu2+ was applied to detect iriothiols. Among the various amino acids, GSH, Cys, and Hcy induced distinct turn-on fluorescence changes. The 1-Cu ensemble was further, applied for GSH detection in living cells.
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