Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
- 주제(키워드) Anaerobic digester , Droplet-digital PCR , mcrA , Methanogen community , Quantitative real-time PCR
- 등재 SCOPUS
- 발행기관 Elsevier
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000115377
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.btre.2014.06.010
초록/요약
Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copiesmL-DNA extract-1). The mcrA-based methanogen communities in three full-scale anaerobic digesters were examined using the two technologies. dd-PCR detected seven groups from the digesters, while qPCR did five groups, indicating that dd-PCR is more sensitive for DNA quantification. Linear regression showed quantitative agreements between both of the technologies (R2 = 0.59-0.98) in the five groups that were concurrently detected. Principal component analysis from the two datasets consistently indicated a substantial difference in the community composition among the digesters and revealed similar levels of differentiation among the communities. The combined results suggest that dd-PCR is more promising for examining methanogenic archaeal communities in biotechnological processes. © 2014 The Authors. Published by Elsevier B.V.
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