Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide
- 주제(키워드) Biofilm , Candida albicans , Sterilization , Supercritical carbon dioxide
- 등재 SCIE, SCOPUS
- 발행기관 Springer Verlag
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000118242
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s00449-015-1414-7
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Present sterilization methods for biofilms in medical devices have limitations. Therefore, an alternative sterilization method using supercritical carbon dioxide (SC-CO<inf>2</inf>) was tested on Candida albicans biofilms. The effect of varying pressure, temperature, and treatment time on the inactivation of C. albicans spores in suspensions and in biofilms was examined. The parameters such as treatment time, pressure, and temperature that led to the complete inactivation of C. albicans biofilms ranged 5–20 min, 100–200 bar, and 35–45 °C, respectively. Notably, treatment of SC-CO<inf>2</inf> at either 100 bar and 40 °C or 200 bar and 30 °C induced complete inactivation of spores within 5 min. Furthermore, it was found that wet biofilms (0.4 %, w/w) had higher sensitivity to SC-CO<inf>2</inf> than dried biofilms. Finally, spore inactivation was confirmed by confocal laser scanning microscopy. In this study, the use of a low-temperature SC-CO<inf>2</inf> sterilization method was proven to be effective in fungal biofilm inactivation, and the moisture content of biofilms was revealed to be the key factor for biofilm inactivation. © 2015 Springer-Verlag Berlin Heidelberg
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