Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV
- 주제(키워드) Eucommiae cortex , HPLC , Pattern recognition , Quality control
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 Pharmaceutical Society of Korea
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000118677
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s12272-015-0642-3
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-β-d-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J’sphere ODS C<inf>18</inf> column (250 × 4.6 mm, 4 µm). The elution was done using water with 0.1 % phosphoric acid (A) and acetonitrile with 0.1 % phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r2 > 0.999) within the test ranges. This method showed good recovery and reproducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53 % (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28 % for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex. © 2015 The Pharmaceutical Society of Korea
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