Cathepsin B Imaging to Predict Quality of Engineered Cartilage
- 주제(키워드) cartilage tissue engineering , cathepsin B , diagnosis , molecular imaging , transplantation
- 등재 SCIE, SCOPUS
- 발행기관 Wiley-VCH Verlag
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000119271
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/mabi.201500215
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
more