HIV-1 tat protein promotes amyloid β generation and tau phosphorylation in rat hippocampal slices
- 주제(키워드) Alzheimer's disease , Aβ , Hippocampus , Hiv-1 tat , Hiv-1-associated neurocognitive disorder , Ptau
- 등재 KCI등재, SCOPUS
- 발행기관 Korean Society for Microbiology/Taehan Misaengmul Hakhoe
- 발행년도 2014
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000119342
- 본문언어 영어
- Published As http://dx.doi.org/10.4167/jbv.2014.44.1.102
초록/요약
HIV-1 Tat protein has been implicated as a causative agent in the pathogenesis of HIV-1-associated neurocognitive disorder (HAND) and Alzheimer's disease (AD)-like pathology in HIV-1 infected patients. Here, we provide insights into the potential roles of extracellular HIV-1 Tat protein in amyloid β (Aβ) generation and Tau phosphorylation, two major neuropathological features of AD. Exposure of the rat hippocampal slices to the full-length HIV-1 Tat protein (Tat1-86) for 3 days led to the increased levels of Aβ precursor protein (APP) accumulation, which accompanied by Aβ generation in the hippocampus, the brain region most commonly damaged in HIV-1-associated dementia (HAD). Moreover, extracellular HIV-1 Tat significantly stimulated the level of phosphrylated Tau (pTau) identified using immunoblotting with AT8 antibody, which recognizes abnormally hyperphosphorylated Tau. Collectively, our data suggest that HIV-1 Tat plays important roles in increasing the levels of APP accumulation, Aβ generation and Tau phosphorylation in the hippocampus, and thereby might contribute to the development of AD-like pathology in HIV-1-infected patients.
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