Transplantation of human spleen into immunodeficient NOD/SCID IL2Rγnull mice generates humanized mice that improve functional B cell development
- 주제(키워드) Bone , CD34+ cells , Hematopoiesis , Humanized mice , Spleen , Thymus
- 등재 SCIE, SCOPUS
- 발행기관 Academic Press Inc.
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000119726
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.clim.2015.09.001
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
We previously generated humanized TB34N mice that received human fetal thymus (T), bone tissue (B) and fetal liver-derived (FL)-CD34+ cells (34) in immunodeficient, NOD/SCID IL2Rγnull (N) mice. Although humanized TB34N mice had excellent hematopoiesis, here, we sought to further improve this model by additional transplantation of human spleen tissue (S) as a secondary hematopoietic tissue (TBS34N). The human spleen grafts were enlarged and differentiated into a similar morphology of adult humans, including follicular lymphoid structures with T- and B-cells. The TBS34N mice mimicked mature human immune system (HIS): mature T- and B-cells and follicular dendritic cells; activated germinal center B-cells expressing CD71, BR3+ cells, memory B-cells and activation-induced cytidine deaminase+ B-cells; CD138+ plasma cells were enriched in the mouse spleen. HBsAg-specific hIgG antibodies were secreted into the sera of all TBS34N mice upon immunization with HBsAg. Taken together, the humanized TBS34N mice improved mature HIS and achieved adaptive antibody responses. © 2015 Elsevier Inc.
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