Proteomic study reveals photosynthesis as downstream of both MAP kinase and cAMP signaling pathways in Chlamydomonas reinhardtii
- 주제(키워드) gene regulation , mass spectrometry , two-dimensional gel electrophoresis
- 등재 SCIE, SCOPUS
- 발행기관 Kluwer Academic Publishers
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000120237
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s11099-015-0128-z
초록/요약
Previously, our data indicated that both cAMP and MAP kinase signaling play important roles in microalgal physiology as well as in lipid or carotenoid biosynthesis. In order to understand downstream genes of these signaling pathways, we employed proteomics approach. Both signal pathways were first altered with specific signaling inhibitors or modulators. Treatment of specific inhibitors changed microalgal size and increased lipid contents. With the microalgal cells after treatments of specific signaling inhibitor or modulators, we performed the proteomics analysis to identify downstream genes responsible for these phenotypes. Interestingly, multiple photosynthesis genes were identified, particularly proteins associated with PSII. Our data suggested that MAP kinase and cAMP signaling affect the photosynthesis, thereby leading to microalgal lipid or carotenoid biosynthesis. © 2015, The Institute of Experimental Botany.
more