Phytosphingosine promotes megakaryocytic differentiation of myeloid leukemia cells
- 주제(키워드) (R)-TEMOSPho , K562 , Megakaryocyte , Phytosphingosine , Platelet
- 등재 SCIE, KCI등재, SCOPUS
- 발행기관 The Biochemical Society of the Republic of Korea
- 발행년도 2015
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000123008
- 본문언어 영어
- Published As http://dx.doi.org/10.5483/BMBRep.2015.48.12.100
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
We report that phytosphingosine, a sphingolipid found in many organisms and implicated in cellular signaling, promotes megakaryocytic differentiation of myeloid leukemia cells. Specifically, phytosphingosine induced several hallmark changes associated with megakaryopoiesis from K562 and HEL cells including cell cycle arrest, cell size increase and polyploidization. We also confirmed that cell type specific markers of megakaryocytes, CD41a and CD42b are induced by phytosphingosine. Phospholipids with highly similar structures were unable to induce similar changes, indicating that the activity of phytosphingosine is highly specific. Although phytosphingosine is known to activate p38 mitogen-activated protein kinase (MAPK)-mediated apoptosis, the signaling mechanisms involved in megakaryopoiesis appear to be distinct. In sum, we present another model for dissecting molecular details of megakaryocytic differentiation which in large part remains obscure. © 2015 by the The Korean Society for Biochemistry and Molecular Biology.
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