Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis
- 주제(키워드) Bacillus subtilis , Bacterial DNA extraction , Low cost , Portable lysis apparatus , Rapid
- 등재 SCIE, SCOPUS
- 발행기관 Blackwell Publishing Ltd
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000123806
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/jam.13011
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5%) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology. © 2016 The Society for Applied Microbiology.
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