Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification
- 주제(키워드) Crypthecodinium cohnii , Docosahexaenoic acid (DHA) , Esterification , Lipase , Microalgae , Rhizomucor miehei
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2016
- URI http://www.dcollection.net/handler/ewha/000000124248
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jff.2015.12.020
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min. © 2015 Elsevier Ltd.
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