A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000130564
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.6b01738
초록/요약
Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells-via two-photon microscopy.
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