Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv
- 주제(키워드) cross-protection , MARTX toxin , monoclonal antibody , Vibrio species
- 등재 SCIE, SCOPUS
- 발행기관 Blackwell Publishing Asia
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000139444
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/1348-0421.12449
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins. © 2016 The Societies and John Wiley & Sons Australia, Ltd
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