A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons
- 주제(키워드) Fluorescent probe , Mitochondria selective probe , Mitochondria transport in neurons , Two photon probe , Viscosity probe
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2016
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000141687
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bios.2016.07.026
초록/요약
We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated. © 2016 Elsevier B.V.
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