In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe
- 주제(키워드) Enzyme-activated fluorescence , Multiple functional probe , NIR bioimaging , Specific phototherapy
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2017
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000141692
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.biomaterials.2017.01.020
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation. © 2017 Elsevier Ltd
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