Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events
- 주제(키워드) Asian dust events , Bacillus subtilis , Biosensor , Carbon nanotubes , Pathogen
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000141877
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.chemosphere.2017.02.060
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102–1010 CFU/mL) and the detection limit (102 CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis. © 2017 Elsevier Ltd
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