Vitamin D Insufficiency Exacerbates Adipose Tissue Macrophage Infiltration and Decreases AMPK/SIRT1 Activity in Obese Rats
- 주제(키워드) adenosine monophosphate-activated protein kinase (AMPK) , adipose tissue macrophage infiltration , obesity , sirtulin 1 (SIRT1) , vitamin D
- 등재 SCIE, SCOPUS
- 발행기관 MDPI AG
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000144769
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/nu9040338
초록/요약
Obesity is recognized as a state of chronic low-grade systemic inflammation due to adipose tissue macrophage infiltration and production of proinflammatory adipokines. Decreased vitamin D status is associated with obesity. The specific aim of the present study is to investigate the effects of vitamin D on obesity-induced adipose tissue inflammation. Male Sprague-Dawley rats were randomized and fed a normal diet (NOR, 1000 IU vitamin D/kg diet), a 45% high-fat diet (HF, 1000 IU vitamin D/kg diet), or a 45% high-fat diet containing 25 IU vitamin D/kg diet (HF+LVD) for 12 weeks. The vitamin D-insufficient diet (HF+LVD) led to vitamin D inadequacy as determined by serum 25(OH) D level, 68.56 +/- 7w.97 nmol/L. The HF+LVD group exacerbated HF-increased adipocyte size, adipogenic gene expression of PPAR gamma, adipose tissue macrophage recruitment, and proinflammatory cytokine IL-6 and TNF alpha levels in epididymal white adipose tissue. In addition, vitamin D insufficiency significantly decreased mRNA levels of beta-oxidation-related genes such as CPT1 alpha, PGC1 alpha, PPAR alpha, VLCAD, LCAD, MCAD, and UCP1. Moreover, significant decrements of SIRT1 and AMPK activity were noted in obese rats fed with a vitamin D-insufficient diet. The observed deleterious effects of vitamin D insufficiency on adipose tissue expansion, immune cell infiltration and inflammatory status suggest vitamin D plays a beneficial role in adipocyte metabolic metabolism and obesity progression. SIRT1 and AMPK activity may play a role in the mechanism of vitamin D action.
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