Aptamer-coded DNA nanoparticles for targeted doxorubicin delivery using pH-sensitive spacer
- 주제(키워드) aptamer , DNA nanoparticles , doxorubicin , drug delivery , pH sensitive , rolling circle amplification
- 등재 SCIE, SCOPUS
- 발행기관 Higher Education Press
- 발행년도 2017
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000145531
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s11705-017-1645-z
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
An anticancer drug delivery system consisting of DNA nanoparticles synthesized by rolling circle amplification (RCA) was developed for prostate cancer membrane antigen (PSMA) targeted cancer therapy. The template of RCA was a DNA oligodeoxynucleotide coded with PSMA-targeted aptamer, drug-loading domain, primer binding site and pH-sensitive spacer. Anticancer drug doxorubicin, as the model drug, was loaded into the drug-loading domain (multiple GC-pair sequences) of the DNA nanoparticles by intercalation. Due to the integrated pH-sensitive spacers in the nanoparticles, in an acidic environment, the cumulative release of doxorubicin was far more than the cumulative release of the drug in the normal physiological environment. In cell uptake experiments, treated with doxorubicin loaded DNA nanoparticles, PSMA-positive C4-2 cells could take up more doxorubicin than PSMA-null PC-3 cells. The prepared DNA nanoparticles showed the potential as drug delivery system for PSMA targeting prostate cancer therapy.[Figure not available: see fulltext.] © 2017 Higher Education Press and Springer-Verlag Berlin Heidelberg
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