Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization
- 주제(키워드) Biosensor , Colocalization , Plasmonic lithography , Sensitivity , Surface plasmon resonance
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2017
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000145609
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.bios.2017.04.046
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection. © 2017 Elsevier B.V.
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